Reporter

Part:BBa_K1053105:Design

Designed by: Eri Takagi   Group: iGEM13_Tokyo-NoKoGen   (2013-09-28)


Pconst.(W)-RBS-FA-linker-MS2-RBS-FB-linker-PP7-double terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1728
    Illegal BglII site found at 1984
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We will combine BBa_K1053110.


Source

We used the synthesized sequences of split GFP fused with MS2 and PP7 (FA-linker-MS2, FB-linker-PP7) respectively, reference of BioBrick part BBa_K738004 (FA-linker-MS2) and BBa_K738005 (FB-linker-PP7) (ZJU-China 2012).

References